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Theoretical Modelling

By GlasgowUni 2011

During our project DISColi, regarding Bio-photolithography in biofilms, we created mathematical models involving Phosphodiesterase(PDE) with VieA domain.

For the model of PDE activity we made several simple calculations and assumptions. The concentration of c-di-GMP in an average bacterial cell is low, 1.25x10^-17 [μmol/cell], the Vmax of PDE is 1.5457x10^-15 [μmol min^-1 molecule^-1]. This means an average cell can produce around 99.6 molecules of PDE per minute. As a result, fully active PDE should be able to deplete a cell of c-di-GMP in ~0.01 minutes (0.6 secs). Therefore, to compensate for any inaccuracies, allowing production of PDE for a full minute should deprive the cell completely of c-di-GMP. Lack of c-di-GMP would increase motility of bacteria and cause biofilm dispersal. Using this in concordance with surfactant protein expression should generate a cumulative effect on dispersal.

For more information, please visit GlasgowUni 2011 Wiki